Intermediate

Part:BBa_I723027

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)

PhzS plus terminator

This is the coding region for the second of two Pseudomonas aeruginosa PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin plus a double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 76
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 283
    Illegal AgeI site found at 382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 385
    Illegal SapI.rc site found at 1146


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Categories
Parameters
None